E8406
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 | Immunoblotting Whole cell extract of HEK-293T expressing human recombinant EDEM1 was separated on SDS-PAGE and blotted with Rabbit Anti-EDEM1 (Cat. No. E8406). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 2 μg/mL + EDEM1 immunizing peptide (human, 629-646 ) 3. Antibody 1 μg/mL 4. Antibody 0.5 μg/mL |
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E8406
Sigma
Anti-EDEM1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-EDEM, Anti-ER degradation enhancer, mannosidase alpha-like 1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 65 kDa (nonglycosylated human and mouse EDEM1) |
| antigen mol wt 75 kDa (glycoslylated human and mouse EDEM1) | |
| species reactivity | human, mouse |
| application(s) | immunoblotting: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1. |
| immunoprecipitation: 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein. | |
| Gene Information | human ... EDEM1(9695) |
| mouse ... Pvalb(19293) | |
| shipped in | dry ice |
| storage temp. | −20°C |
